ID	SACE_0724	Type	CDS
From	810727	nt length	9516
To	820242	aa length	3171
Gene	eryAIII	pI	4.72
Definition	EryAIII Erythromycin polyketide synthase modules 5 and 6	M.W (Da)	332259
Note		AseI frg.	B
SignalP v.4.0	Non-secretory protein | plot	Category

Orthologs (if any)

Pfam search result

	Model	No.	Description	Position (aa)	Score	E-value
	Acyl_transf_1	2	Acyl transferase domain	556-872, 2017-2329	594.4	5.6e-179
	ketoacyl-synt	2	Beta-ketoacyl synthase, N-terminal domain	39-277, 1486-1735	560.4	4.4e-169
	KR	2	KR domain	1116-1292, 2556-2730	366.9	1.5e-110
	Ketoacyl-synt_C	2	Beta-ketoacyl synthase, C-terminal domain	285-402, 1743-1858	282.6	1.8e-85
	Thioesterase	1	Thioesterase domain	2959-3165	139.2	2e-40
	adh_short	2	short chain dehydrogenase	1117-1282, 2557-2717	127.9	2.9e-37
	KAsynt_C_assoc	2	Ketoacyl-synthetase C-terminal extension	405-520, 1865-1980	122.6	9.1e-36
	PP-binding	2	Phosphopantetheine attachment site	1395-1459, 2828-2889	94.9	2.4e-27
	adh_short_C2	1	Enoyl-(Acyl carrier protein) reductase	1125-1274	75.6	3.8e-21
	Epimerase	2	NAD dependent epimerase/dehydratase family	1118-1250, 2558-2687	55.7	4.3e-15
	Thiolase_N	1	Thiolase, N-terminal domain	165-231	53.9	1.4e-14
	Polysacc_synt_2	2	Polysaccharide biosynthesis protein	1118-1248, 2559-2699	45.9	3.5e-12
	3Beta_HSD	1	3-beta hydroxysteroid dehydrogenase/isomerase family	1119-1253	42.4	3.6e-11
	Abhydrolase_6	1	Alpha/beta hydrolase family	2960-3132	29.3	1.1e-06
	Docking	1	Erythronolide synthase docking	8-32	23.0	4.1e-05

BLAST search result (top 10 hits)

ID	Description	Score	E-value
WP_011873139.1	erythronolide synthase [Saccharopolyspora erythraea] >gb	14142	0.0e+00
AAA26495.1	ORF3 encoding modules 5 & 6 for 6-deoxyerythronolide B formation;...	14122	0.0e+00
Q03133.4	RecName: Full=6-deoxyerythronolide-B synthase EryA3, modules 5 and ...	13706	0.0e+00
CAA39583.1	6-deoxyerythronolide B synthase [Saccharopolyspora erythraea NRR...	13502	0.0e+00
	1702361A polyketide synthase [Saccharopolyspora erythraea]	13496	0.0e+00
AAG13919.1	megalomicin 6-deoxyerythronolide B synthase 3 [Micromonospora meg...	10226	0.0e+00
WP_067345566.1	hypothetical protein [Streptomyces noursei] >gb	8630	0.0e+00
WP_011113114.1	lankamycin synthase LkmAIII [Streptomyces rochei] >ref	8040	0.0e+00
AAU93805.2	polyketide synthase modules 5 and 6 [Aeromicrobium erythreum]	7214	0.0e+00
WP_083516503.1	hypothetical protein [Aeromicrobium erythreum] >gb	7209	0.0e+00

PSORT result

Localization	Certainty	PSORT said
Not Clear

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