| ID | SAVERM_1666
| Type | CDS
|
| From | 2038721
| nt length | 636
|
| To | 2038086
| aa length | 211
|
| Gene | thiJ
| pI | 4.58
|
| Definition | putative 4-methyl-5(B-hydroxyethyl)-thiazole monophosphate biosynthesis enzyme
| M.W (Da) | 22159
|
| Note | PF01965: DJ-1/PfpI family
| AseI frg. | C
|
| SignalP v.4.0 | Non-secretory protein | plot
| Category | metabolism of coenzymes & prosthetic groups
|
Orthologs (if any)
| Bacteria* | Reciprocally best hit to | Score | E-value
|
| coel | SCO6747 hypothetical protein | 306 | 6.0e-108
|
| sgr | SGR_981 conserved hypothetical protein | 326 | 1.0e-115
|
| sam | SAM23877_6397 Isonitrile hydratase | 353 | 2.0e-126
|
| scab | SCAB13141 putative 4-methyl-5(B-hydroxyethyl)-thiazole monophosphate biosynthesis enzyme | 344 | 6.0e-123
|
| sace | SACE_6421 4-methyl-5(B-hydroxyethyl)-thiazole monophosphate biosynthesis enzyme | 200 | 4.0e-66
|
| nfar | nfa50430 hypothetical protein | 194 | 6.3e-51
|
| mtub | gi|15607194 hypothetical protein Rv0052 [Mycobacterium tuberculosis H37Rv] | 156 | 8.6e-40
|
*coel, Streptomyces coelicolor A3(2); sgr, Streptomyces griseus IFO13350; sam, Streptomyces ambofaciens ATCC 23877; scab, Streptomyces scabies 87.22; sace, Saccharopolyspora erythraea NRRL2338; nfar, Nocardia farcinica IFM 10152; mtub, Mycobacterium tuberculosis H37Rv;
Pfam search result
| Model | No. | Description | Position (aa) | Score | E-value
|
| DJ-1_PfpI | 1 | DJ-1/PfpI family | 2-162 | 94.5 | 5.5e-27
|
BLAST search result (top 10 hits)
| ID | Description | Score | E-value
|
| WP_086172594.1 | DJ-1/PfpI family protein [Streptomyces glaucescens] >gb
| 916 | 4.5e-124 |
| WP_053849004.1 | DJ-1/PfpI family protein [Streptomyces sp. NRRL B-24085]
| 913 | 1.2e-123 |
|
| 911 | 2.2e-123 |
| WP_073935029.1 | DJ-1/PfpI family protein [Streptomyces sp. CB02400] >gb
| 911 | 3.3e-123 |
| WP_189314983.1 | DJ-1/PfpI family protein [Streptomyces brasiliensis] >dbj
| 910 | 4.5e-123 |
| WP_109494642.1 | DJ-1/PfpI family protein [Streptomyces sp. Act143] >gb
| 908 | 8.5e-123 |
| WP_030041980.1 | DJ-1/PfpI family protein [Streptomyces resistomycificus] >gb...
| 906 | 1.3e-122 |
| WP_138042916.1 | DJ-1/PfpI family protein [Streptomyces montanus] >lcl
| 905 | 2.1e-122 |
| WP_212670424.1 | DJ-1/PfpI family protein [Streptomyces sp. A2-16] >gb
| 904 | 2.6e-122 |
| WP_028798905.1 | DJ-1/PfpI family protein [Streptomyces sp. 142MFCol3.1]
| 903 | 3.5e-122 |
PSORT result
| Localization | Certainty | PSORT said
|
| cytoplasm | 0.0983 | Seems to have no N-terminal signal seq.
|