| ID | SAVERM_1680
| Type | CDS
|
| From | 2058635
| nt length | 2208
|
| To | 2056428
| aa length | 735
|
| Gene | fadB1
| pI | 5.49
|
| Definition | putative fatty acid oxidation complex alpha-subunit
| M.W (Da) | 78109
|
| Note | similar to glyoxysomal fatty acid beta-oxidation multifunctional protein MFP-a: including enoyl-CoA hydratase; 3-2-trans-enoyl-CoA isomerase; 3-hydroxybutyryl-CoA epimerase; 3-hydroxyacyl-CoA dehydrogenase
| AseI frg. | C
|
| SignalP v.4.0 | Non-secretory protein | plot
| Category | metabolism of lipids
|
Orthologs (if any)
| Bacteria* | Reciprocally best hit to | Score | E-value
|
| coel | SCO6732 possible fatty acid oxidative multifunctional enzyme | 1248 | 0.0e+00
|
| sgr | SGR_993 putative fatty fatty acid oxidation complex alpha-subunit | 1262 | 0.0e+00
|
| sam | SAM23877_6384 Fatty acid oxidative multifunctional enzyme | 1252 | 0.0e+00
|
| scab | SCAB13351 putative fatty acid oxidation complex alpha-subunit | 1235 | 0.0e+00
|
| sace | SACE_6362 putative 3-hydroxyacyl-CoA dehydrogenase | 971 | 0.0e+00
|
| nfar | nfa2860 fadB 3-hydroxyacyl-CoA dehydrogenase | 907 | 0.0e+00
|
| mtub | gi|15608000 PROBABLE FATTY OXIDATION PROTEIN FADB [Mycobacterium tuberculosis H37Rv] | 927 | 0.0e+00
|
| ecoli | gi|16130274 bifunctional fatty acid oxidation complex protein: putative enoyl-CoA hydratase/isomerase (N-terminal); putative NAD(P)-binding dehydrogenase (C-terminal) [Escherichia coli K12] | 335 | 8.2e-93
|
| paer | PA1737 [gene=PA1737] [prot=probable 3-hydroxyacyl-CoA dehydrogenase] [comment=PA1737] | 810 | 0.0e+00
|
*coel, Streptomyces coelicolor A3(2); sgr, Streptomyces griseus IFO13350; sam, Streptomyces ambofaciens ATCC 23877; scab, Streptomyces scabies 87.22; sace, Saccharopolyspora erythraea NRRL2338; nfar, Nocardia farcinica IFM 10152; mtub, Mycobacterium tuberculosis H37Rv; ecoli, Escherichia coli K-12; paer, Pseudomonas aeruginosa;
Pfam search result
| Model | No. | Description | Position (aa) | Score | E-value
|
| 3HCDH_N | 1 | 3-hydroxyacyl-CoA dehydrogenase, NAD binding domain | 323-501 | 227.6 | 8.7e-68
|
| ECH_1 | 1 | Enoyl-CoA hydratase/isomerase | 14-205 | 106.1 | 1.7e-30
|
| 3HCDH | 1 | 3-hydroxyacyl-CoA dehydrogenase, C-terminal domain | 504-604 | 69.4 | 2.9e-19
|
| ECH_2 | 1 | Enoyl-CoA hydratase/isomerase | 20-164 | 59.4 | 3.9e-16
|
BLAST search result (top 10 hits)
| ID | Description | Score | E-value
|
| WP_055494142.1 | 3-hydroxyacyl-CoA dehydrogenase NAD-binding domain-containin...
| 3309 | 0.0e+00 |
| WP_099919521.1 | 3-hydroxyacyl-CoA dehydrogenase NAD-binding domain-containin...
| 3299 | 0.0e+00 |
| WP_099502404.1 | MULTISPECIES: 3-hydroxyacyl-CoA dehydrogenase NAD-binding do...
| 3298 | 0.0e+00 |
| WP_217571973.1 | 3-hydroxyacyl-CoA dehydrogenase NAD-binding domain-containin...
| 3294 | 0.0e+00 |
| WP_189185905.1 | 3-hydroxyacyl-CoA dehydrogenase NAD-binding domain-containin...
| 3294 | 0.0e+00 |
| WP_067361363.1 | 3-hydroxyacyl-CoA dehydrogenase NAD-binding domain-containin...
| 3294 | 0.0e+00 |
| WP_144316068.1 | 3-hydroxyacyl-CoA dehydrogenase NAD-binding domain-containin...
| 3292 | 0.0e+00 |
| WP_075030380.1 | 3-hydroxyacyl-CoA dehydrogenase NAD-binding domain-containin...
| 3292 | 0.0e+00 |
| WP_143656319.1 | 3-hydroxyacyl-CoA dehydrogenase NAD-binding domain-containin...
| 3290 | 0.0e+00 |
| WP_181648963.1 | 3-hydroxyacyl-CoA dehydrogenase NAD-binding domain-containin...
| 3290 | 0.0e+00 |
PSORT result
| Localization | Certainty | PSORT said
|
| cytoplasm | 0.0307 | Seems to have no N-terminal signal seq.
|