| ID | SAVERM_2444
| Type | CDS
|
| From | 2978393
| nt length | 1539
|
| To | 2976855
| aa length | 512
|
| Gene | rpoD
| pI | 5.92
|
| Definition | RNA polymerase major sigma factor, sigma-70 family
| M.W (Da) | 55911
|
| Note | hrdB; sigA, PF04539: Sigma-70 region 3
| AseI frg. | Q
|
| SignalP v.4.0 | Non-secretory protein | plot
| Category | RNA initiation
|
Orthologs (if any)
| Bacteria* | Reciprocally best hit to | Score | E-value
|
| coel | SCO5820 major vegetative sigma factor rpoD | 877 | 0.0e+00
|
| sgr | SGR_1701 RNA polymerase principal sigma factor hrdB | 824 | 0.0e+00
|
| sam | SAM23877_5527 RNA polymerase principal sigma factor HrdB | 884 | 0.0e+00
|
| scab | SCAB24441 RNA polymerase major sigma factor, sigma-70 family | 840 | 0.0e+00
|
| sace | SACE_1801 RNA polymerase major sigma factor | 533 | 0.0e+00
|
| nfar | nfa37590 sigA principal sigma factor | 537 | 9.6e-154
|
| mtub | gi|15609840 RNA polymerase sigma factor [Mycobacterium tuberculosis H37Rv] | 504 | 4.0e-144
|
| cglu | gi|19553115 COG0568:DNA-directed RNA polymerase sigma subunits (sigma70/sigma32) [Corynebacterium glutamicum] | 523 | 1.0e-149
|
| bsub | BG10314 sigA, rpoD, crsA; RNA polymerase major sigma factor | 374 | 7.2e-105
|
| ecoli | gi|16130963 RNA polymerase sigma factor [Escherichia coli K12] | 310 | 1.4e-85
|
| paer | PA0576 [gene=rpoD] [prot=sigma factor RpoD] [comment=PA0576] | 308 | 9.9e-85
|
*coel, Streptomyces coelicolor A3(2); sgr, Streptomyces griseus IFO13350; sam, Streptomyces ambofaciens ATCC 23877; scab, Streptomyces scabies 87.22; sace, Saccharopolyspora erythraea NRRL2338; nfar, Nocardia farcinica IFM 10152; mtub, Mycobacterium tuberculosis H37Rv; cglu, Corynebacterium glutamicum; bsub, Bacillus subtilis; ecoli, Escherichia coli K-12; paer, Pseudomonas aeruginosa;
Pfam search result
| Model | No. | Description | Position (aa) | Score | E-value
|
| Sigma70_r3 | 1 | Sigma-70 region 3 | 358-434 | 100.8 | 3.1e-29
|
| Sigma70_r2 | 1 | Sigma-70 region 2 | 279-348 | 79.0 | 1.6e-22
|
| Sigma70_r4 | 1 | Sigma-70, region 4 | 447-500 | 69.5 | 1.1e-19
|
| Sigma70_r1_2 | 1 | Sigma-70 factor, region 1.2 | 214-247 | 62.7 | 2.1e-17
|
BLAST search result (top 10 hits)
| ID | Description | Score | E-value
|
| WP_234513643.1 | RNA polymerase sigma factor [Streptomyces glomeratus] >lcl
| 1994 | 0.0e+00 |
| WP_189774589.1 | RNA polymerase sigma factor [Streptomyces tauricus] >dbj
| 1985 | 0.0e+00 |
| PSM38263.1 | RNA polymerase sigma factor [Streptomyces dioscori]
| 1977 | 0.0e+00 |
| WP_210889229.1 | RNA polymerase sigma factor [Streptomyces sp. BH-SS-21] >lcl...
| 1975 | 0.0e+00 |
| WP_225891484.1 | RNA polymerase sigma factor [Streptomyces dioscori]
| 1972 | 0.0e+00 |
| WP_111002992.1 | RNA polymerase sigma factor [Streptomyces sp. NTH33] >gb
| 1969 | 0.0e+00 |
| WP_053760824.1 | RNA polymerase sigma factor [Streptomyces cadmiisoli] >gb
| 1968 | 0.0e+00 |
| WP_220292015.1 | RNA polymerase sigma factor [Streptomyces sp. MBT84] >lcl
| 1968 | 0.0e+00 |
| WP_234388903.1 | RNA polymerase sigma factor [Streptomyces sp. AS58] >gb
| 1962 | 0.0e+00 |
| GGR23857.1 | RNA polymerase principal sigma factor HrdB [Streptomyces auranti...
| 1960 | 0.0e+00 |
PSORT result
| Localization | Certainty | PSORT said
|
| outside | 0.3000 | Seems to have a cleavable N-term signal seq.
|