| ID | SAVERM_2479
| Type | CDS
|
| From | 3027844
| nt length | 1527
|
| To | 3026318
| aa length | 508
|
| Gene | miaB
| pI | 5.86
|
| Definition | putative methylase of isopentenylated A37 derivatives in tRNA
| M.W (Da) | 55716
|
| Note | PF00919: Uncharacterized protein family UPF0004
| AseI frg. | Q
|
| SignalP v.4.0 | Non-secretory protein | plot
| Category | RNA modification
|
Orthologs (if any)
| Bacteria* | Reciprocally best hit to | Score | E-value
|
| coel | SCO5787 hypothetical protein | 957 | 0.0e+00
|
| sgr | SGR_1732 conserved hypothetical protein | 927 | 0.0e+00
|
| sam | SAM23877_5490 (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB | 946 | 0.0e+00
|
| scab | SCAB24811 putative methylase of isopentenylated A37 derivatives in tRNA | 924 | 0.0e+00
|
| sace | SACE_1748 methylase of isopentenylated A37 derivatives in | 733 | 0.0e+00
|
| nfar | nfa38350 hypothetical protein | 734 | 0.0e+00
|
| mtub | gi|15609870 CONSERVED HYPOTHETICAL ALANINE, ARGININE-RICH PROTEIN [Mycobacterium tuberculosis H37Rv] | 674 | 0.0e+00
|
| cglu | gi|19553154 COG0621:2-methylthioadenine synthetase [Corynebacterium glutamicum] | 669 | 0.0e+00
|
| bsub | BG13418 ymcB; methylation of Isopentenylated A37 derivatives in tRNA | 358 | 4.0e-100
|
| ecoli | gi|16128644 involved in methylthiolation of isopentenylated A37 derivatives in tRNA [Escherichia coli K12] | 320 | 1.1e-88
|
| paer | PA3980 [gene=PA3980] [prot=conserved hypothetical protein] [comment=PA3980] | 320 | 1.9e-88
|
*coel, Streptomyces coelicolor A3(2); sgr, Streptomyces griseus IFO13350; sam, Streptomyces ambofaciens ATCC 23877; scab, Streptomyces scabies 87.22; sace, Saccharopolyspora erythraea NRRL2338; nfar, Nocardia farcinica IFM 10152; mtub, Mycobacterium tuberculosis H37Rv; cglu, Corynebacterium glutamicum; bsub, Bacillus subtilis; ecoli, Escherichia coli K-12; paer, Pseudomonas aeruginosa;
Pfam search result
| Model | No. | Description | Position (aa) | Score | E-value
|
| Radical_SAM | 1 | Radical SAM superfamily | 162-332 | 101.1 | 7.5e-29
|
| UPF0004 | 1 | Uncharacterized protein family UPF0004 | 15-115 | 93.4 | 6.1e-27
|
BLAST search result (top 10 hits)
| ID | Description | Score | E-value
|
| NUR01585.1 | tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase MiaB...
| 2583 | 0.0e+00 |
| WP_232655291.1 | tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase...
| 2575 | 0.0e+00 |
| WP_095937064.1 | tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase...
| 2572 | 0.0e+00 |
| WP_189188202.1 | tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase...
| 2570 | 0.0e+00 |
| WP_028802329.1 | tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase...
| 2568 | 0.0e+00 |
| WP_201048151.1 | tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase...
| 2568 | 0.0e+00 |
| WP_123967890.1 | tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase...
| 2567 | 0.0e+00 |
| WP_200709683.1 | tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase...
| 2565 | 0.0e+00 |
| WP_030947258.1 | tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase...
| 2564 | 0.0e+00 |
| WP_093629534.1 | tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase...
| 2562 | 0.0e+00 |
PSORT result
| Localization | Certainty | PSORT said
|
| cytoplasm | 0.2867 | Seems to have no N-terminal signal seq.
|