| ID | SAVERM_2641
| Type | CDS
|
| From | 3239375
| nt length | 849
|
| To | 3238527
| aa length | 282
|
| Gene | trmD
| pI | 5.11
|
| Definition | putative tRNA (guanine-N(1)-)-methyltransferase
| M.W (Da) | 31637
|
| Note | PF01746: tRNA (Guanine-1)-methyltransferase
| AseI frg. | J
|
| SignalP v.4.0 | Non-secretory protein | plot
| Category | RNA modification
|
Orthologs (if any)
| Bacteria* | Reciprocally best hit to | Score | E-value
|
| coel | SCO5594 tRNA (guanine-N1)-methyltransferase | 510 | 0.0e+00
|
| sgr | SGR_1886 putative RNA (guanine-N1)-methyltransferase | 475 | 2.0e-172
|
| sam | SAM23877_5350 tRNA (guanine-1-)-methyltransferase | 510 | 0.0e+00
|
| scab | SCAB26161 putative tRNA (guanine-N(1)-)-methyltransferase | 506 | 0.0e+00
|
| sace | SACE_6050 tRNA (guanine-N(1)-)-methyltransferase | 295 | 2.0e-101
|
| nfar | nfa41540 trmD tRNA methyltransferase | 277 | 8.6e-76
|
| mtub | gi|15610043 tRNA (guanine-N(1)-)-methyltransferase [Mycobacterium tuberculosis H37Rv] | 258 | 3.2e-70
|
| cglu | gi|19553251 COG0336:tRNA-(guanine-N1)-methyltransferase [Corynebacterium glutamicum] | 274 | 3.9e-75
|
| bsub | BG12687 trmD; tRNA methyltransferase [EC:2.1.1.31] | 188 | 3.5e-49
|
| ecoli | gi|16130528 tRNA (guanine-N(1)-)-methyltransferase [Escherichia coli K12] | 173 | 1.0e-44
|
| paer | PA3743 [gene=trmD] [prot=tRNA (guanine-N1)-methyltransferase] [comment=PA3743] | 187 | 1.2e-48
|
*coel, Streptomyces coelicolor A3(2); sgr, Streptomyces griseus IFO13350; sam, Streptomyces ambofaciens ATCC 23877; scab, Streptomyces scabies 87.22; sace, Saccharopolyspora erythraea NRRL2338; nfar, Nocardia farcinica IFM 10152; mtub, Mycobacterium tuberculosis H37Rv; cglu, Corynebacterium glutamicum; bsub, Bacillus subtilis; ecoli, Escherichia coli K-12; paer, Pseudomonas aeruginosa;
Pfam search result
| Model | No. | Description | Position (aa) | Score | E-value
|
| tRNA_m1G_MT | 1 | tRNA (Guanine-1)-methyltransferase | 27-237 | 244.6 | 5.9e-73
|
BLAST search result (top 10 hits)
| ID | Description | Score | E-value
|
| WP_099502620.1 | MULTISPECIES: tRNA (guanosine(37)-N1)-methyltransferase TrmD...
| 1355 | 0.0e+00 |
| WP_210588103.1 | tRNA (guanosine(37)-N1)-methyltransferase TrmD [Streptomyces...
| 1351 | 0.0e+00 |
| WP_217547451.1 | tRNA (guanosine(37)-N1)-methyltransferase TrmD [Streptomyces...
| 1350 | 0.0e+00 |
| WP_059198034.1 | tRNA (guanosine(37)-N1)-methyltransferase TrmD [Streptomyces...
| 1346 | 0.0e+00 |
| WP_138049235.1 | tRNA (guanosine(37)-N1)-methyltransferase TrmD [Streptomyces...
| 1346 | 0.0e+00 |
| NUS26013.1 | tRNA (guanosine(37)-N1)-methyltransferase TrmD [Streptomyces sp.]
| 1346 | 0.0e+00 |
| WP_109495723.1 | tRNA (guanosine(37)-N1)-methyltransferase TrmD [Streptomyces...
| 1345 | 0.0e+00 |
| OPG06067.1 | tRNA (guanosine(37)-N1)-methyltransferase TrmD [Streptomyces sp. ...
| 1345 | 0.0e+00 |
| WP_194084849.1 | tRNA (guanosine(37)-N1)-methyltransferase TrmD [Streptomyces...
| 1345 | 0.0e+00 |
| NUO45483.1 | tRNA (guanosine(37)-N1)-methyltransferase TrmD [Streptomyces sp.]
| 1344 | 0.0e+00 |
PSORT result
| Localization | Certainty | PSORT said
|
| membrane | 0.1914 | Seems to have no N-terminal signal seq.
|