| ID | SAVERM_2685
| Type | CDS
|
| From | 3291557
| nt length | 594
|
| To | 3290964
| aa length | 197
|
| Gene | leuD
| pI | 5.15
|
| Definition | putative 3-isopropylmalate dehydratase subunit small
| M.W (Da) | 22161
|
| Note | PF00694: Aconitase C-terminal domain
| AseI frg. | J
|
| SignalP v.4.0 | Non-secretory protein | plot
| Category | metabolism of amino acids & related molecules
|
Orthologs (if any)
| Bacteria* | Reciprocally best hit to | Score | E-value
|
| coel | SCO5554 3-isopropylmalate dehydratase small subunit | 370 | 1.0e-133
|
| sgr | SGR_1928 putative 3-isopropylmalate dehydratase small subunit | 369 | 6.0e-133
|
| sam | SAM23877_5306 3-isopropylmalate isomerase subunit | 371 | 5.0e-134
|
| scab | SCAB26601 putative 3-isopropylmalate dehydratase subunit small | 374 | 4.0e-135
|
| sace | SACE_6143 3-isopropylmalate dehydratase small subunit | 258 | 4.0e-89
|
| nfar | nfa42110 leuD isopropylmalate isomerase subunit | 238 | 2.0e-64
|
| mtub | gi|15610124 isopropylmalate isomerase small subunit [Mycobacterium tuberculosis H37Rv] | 227 | 4.7e-61
|
| cglu | gi|19552535 COG0066:3-isopropylmalate dehydratase small subunit [Corynebacterium glutamicum] | 240 | 3.8e-65
|
| bsub | BG11950 leuD; 3-isopropylmalate dehydratase small subunit [EC:4.2.1.33] | 150 | 6.4e-38
|
| ecoli | gi|16128065 isopropylmalate isomerase small subunit [Escherichia coli K12] | 177 | 4.2e-46
|
| paer | PA3120 [gene=leuD] [prot=3-isopropylmalate dehydratase small subunit] [comment=PA3120] | 162 | 1.5e-41
|
*coel, Streptomyces coelicolor A3(2); sgr, Streptomyces griseus IFO13350; sam, Streptomyces ambofaciens ATCC 23877; scab, Streptomyces scabies 87.22; sace, Saccharopolyspora erythraea NRRL2338; nfar, Nocardia farcinica IFM 10152; mtub, Mycobacterium tuberculosis H37Rv; cglu, Corynebacterium glutamicum; bsub, Bacillus subtilis; ecoli, Escherichia coli K-12; paer, Pseudomonas aeruginosa;
Pfam search result
| Model | No. | Description | Position (aa) | Score | E-value
|
| Aconitase_C | 1 | Aconitase C-terminal domain | 1-117 | 92.0 | 3.4e-26
|
BLAST search result (top 10 hits)
| ID | Description | Score | E-value
|
| WP_033282691.1 | 3-isopropylmalate dehydratase small subunit [Streptomyces sp...
| 1002 | 1.3e-137 |
| WP_189937420.1 | 3-isopropylmalate dehydratase small subunit [Streptomyces au...
| 999 | 3.8e-137 |
| WP_019058654.1 | 3-isopropylmalate dehydratase small subunit [Streptomyces pr...
| 997 | 6.4e-137 |
| WP_054235401.1 | MULTISPECIES: 3-isopropylmalate dehydratase small subunit [u...
| 996 | 1.0e-136 |
| WP_019068723.1 | MULTISPECIES: 3-isopropylmalate dehydratase small subunit [S...
| 995 | 1.2e-136 |
| WP_043680410.1 | 3-isopropylmalate dehydratase small subunit [Streptomyces xy...
| 994 | 1.8e-136 |
| WP_200713349.1 | MULTISPECIES: 3-isopropylmalate dehydratase small subunit [u...
| 992 | 4.1e-136 |
| WP_124438971.1 | 3-isopropylmalate dehydratase small subunit [Streptomyces sp...
| 991 | 4.7e-136 |
| WP_107021934.1 | 3-isopropylmalate dehydratase small subunit [Streptomyces di...
| 991 | 4.8e-136 |
| WP_150951311.1 | 3-isopropylmalate dehydratase small subunit [Streptomyces lu...
| 991 | 5.9e-136 |
PSORT result
| Localization | Certainty | PSORT said
|
| cytoplasm | 0.4211 | Seems to have no N-terminal signal seq.
|