| ID | SAVERM_2710
| Type | CDS
|
| From | 3325518
| nt length | 1605
|
| To | 3323914
| aa length | 534
|
| Gene | leuA1
| pI | 5
|
| Definition | putative 2-isopropylmalate synthase
| M.W (Da) | 57657
|
| Note | PF00682: HMGL-like
| AseI frg. | J
|
| SignalP v.4.0 | Non-secretory protein | plot
| Category | metabolism of amino acids & related molecules
|
Orthologs (if any)
| Bacteria* | Reciprocally best hit to | Score | E-value
|
| coel | SCO5529 putative lyase | 1013 | 0.0e+00
|
| sgr | SGR_1979 putative 2-isopropylmalate synthase | 974 | 0.0e+00
|
| sam | SAM23877_5281 putative citramalate synthase | 1006 | 0.0e+00
|
| scab | SCAB26871 putative 2-isopropylmalate synthase | 1019 | 0.0e+00
|
| sace | SACE_6151 2-isopropylmalate synthase 2 | 639 | 0.0e+00
|
| nfar | nfa4670 4-hydroxy-2-oxovalerate aldolase | 70 | 3.5e-13
|
| mtub | gi|15610605 4-hydroxy-2-ketovalerate aldolase [Mycobacterium tuberculosis H37Rv] | 72 | 9.2e-14
|
| bsub | BG11948 leuA; 2-isopropylmalate synthase [EC:4.1.3.12] | 157 | 1.1e-39
|
| ecoli | gi|16128068 2-isopropylmalate synthase [Escherichia coli K12] | 163 | 3.0e-41
|
| paer | PA1217 [gene=PA1217] [prot=probable 2-isopropylmalate synthase] [comment=PA1217] | 115 | 1.3e-26
|
*coel, Streptomyces coelicolor A3(2); sgr, Streptomyces griseus IFO13350; sam, Streptomyces ambofaciens ATCC 23877; scab, Streptomyces scabies 87.22; sace, Saccharopolyspora erythraea NRRL2338; nfar, Nocardia farcinica IFM 10152; mtub, Mycobacterium tuberculosis H37Rv; bsub, Bacillus subtilis; ecoli, Escherichia coli K-12; paer, Pseudomonas aeruginosa;
Pfam search result
| Model | No. | Description | Position (aa) | Score | E-value
|
| HMGL-like | 1 | HMGL-like | 12-280 | 158.3 | 2.4e-46
|
| LeuA_dimer | 1 | LeuA allosteric (dimerisation) domain | 382-523 | 94.4 | 4.7e-27
|
BLAST search result (top 10 hits)
PSORT result
| Localization | Certainty | PSORT said
|
| cytoplasm | 0.3526 | Seems to have no N-terminal signal seq.
|