| ID | SAVERM_3117
| Type | CDS
|
| From | 3891882
| nt length | 660
|
| To | 3891223
| aa length | 219
|
| Gene | sig23
| pI | 7.3
|
| Definition | putative RNA polymerase ECF-subfamily sigma factor
| M.W (Da) | 24216
|
| Note | similaro to SCO5147, PF04542: Sigma-70 region 2
| AseI frg. | E
|
| SignalP v.4.0 | Non-secretory protein | plot
| Category | RNA initiation
|
Orthologs (if any)
| Bacteria* | Reciprocally best hit to | Score | E-value
|
| coel | SCO5147 putative ECF-subfamily sigma factor | 392 | 1.0e-141
|
| sgr | SGR_2378 putative RNA polymerase ECF-subfamily sigma factor | 385 | 5.0e-139
|
| sam | SAM23877_4926 RNA polymerase sigma factor SigE | 391 | 1.0e-140
|
| scab | SCAB31151 putative RNA polymerase sigma factor | 403 | 1.0e-145
|
| sace | SACE_1037 putative ECF family RNA polymerase sigma factor | 253 | 7.0e-87
|
| nfar | nfa47290 sigma factor | 250 | 6.0e-68
|
| mtub | gi|15608361 RNA polymerase sigma-70 factor [Mycobacterium tuberculosis H37Rv] | 243 | 9.7e-66
|
| cglu | gi|19552346 COG1595:DNA-directed RNA polymerase specialized sigma subunits, sigma24 homologs [Corynebacterium glutamicum] | 227 | 3.0e-61
|
| bsub | BG11573 sigW, ybbL; RNA polymerase ECF-type sigma factor | 97 | 5.8e-22
|
*coel, Streptomyces coelicolor A3(2); sgr, Streptomyces griseus IFO13350; sam, Streptomyces ambofaciens ATCC 23877; scab, Streptomyces scabies 87.22; sace, Saccharopolyspora erythraea NRRL2338; nfar, Nocardia farcinica IFM 10152; mtub, Mycobacterium tuberculosis H37Rv; cglu, Corynebacterium glutamicum; bsub, Bacillus subtilis;
Pfam search result
| Model | No. | Description | Position (aa) | Score | E-value
|
| Sigma70_r2 | 1 | Sigma-70 region 2 | 37-102 | 73.8 | 6.3e-21
|
| Sigma70_r4_2 | 1 | Sigma-70, region 4 | 135-186 | 61.1 | 5.6e-17
|
| Sigma70_r4 | 1 | Sigma-70, region 4 | 139-188 | 49.0 | 2.9e-13
|
BLAST search result (top 10 hits)
| ID | Description | Score | E-value
|
| WP_202497427.1 | RNA polymerase sigma factor SigE [Streptomyces sp. SID5469]
| 1037 | 1.3e-141 |
| KPI21329.1 | RNA polymerase, sigma-24 subunit, RpoE, ECF subfamily [Actinobact...
| 1023 | 4.2e-140 |
| WP_099499720.1 | MULTISPECIES: RNA polymerase sigma factor SigE [Streptomyces...
| 1021 | 1.4e-139 |
| WP_054228218.1 | MULTISPECIES: RNA polymerase sigma factor SigE [Streptomyces...
| 1019 | 2.9e-139 |
| WP_168495278.1 | MULTISPECIES: RNA polymerase sigma factor SigE [Streptomyces...
| 1019 | 3.1e-139 |
| WP_211180119.1 | RNA polymerase sigma factor SigE [Streptomyces sp. RLA2-12]
| 1017 | 1.1e-138 |
| WP_168530085.1 | RNA polymerase sigma factor SigE [Streptomyces sp. RPA4-2] >...
| 1010 | 7.2e-138 |
| WP_095936463.1 | RNA polymerase sigma factor SigE [Streptomyces sp. Tue6028] ...
| 1006 | 2.9e-137 |
| WP_151479039.1 | RNA polymerase sigma factor SigE [Streptomyces albicerus]
| 1004 | 6.0e-137 |
| NSL43804.1 | RNA polymerase sigma factor SigE [Streptomyces sp. 8P21H-1]
| 1003 | 6.3e-137 |
PSORT result
| Localization | Certainty | PSORT said
|
| cytoplasm | 0.1569 | Seems to have no N-terminal signal seq.
|