| ID | SAVERM_3586
| Type | CDS
|
| From | 4440294
| nt length | 894
|
| To | 4439401
| aa length | 297
|
| Gene | ispE
| pI | 4.37
|
| Definition | putative 4-(cytidine-5’-diphospho)-2-C-methyl-D-erythritol kinase
| M.W (Da) | 29713
|
| Note | MEP pathway, EC 2.7.1.148
| AseI frg. | E
|
| SignalP v.4.0 | Non-secretory protein | plot
| Category | specific pathways
|
Orthologs (if any)
| Bacteria* | Reciprocally best hit to | Score | E-value
|
| coel | SCO3148 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase | 381 | 1.0e-134
|
| sgr | SGR_4357 putative 4-(cytidine-5-diphospho)-2-C-methyl-D- erythritol kinase | 415 | 7.0e-148
|
| sam | SAM23877_3195 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase | 416 | 2.0e-148
|
| scab | SCAB53871 putative 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase | 422 | 1.0e-150
|
| sace | SACE_0807 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase | 227 | 5.0e-74
|
| nfar | nfa49010 cmek 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase | 295 | 2.5e-81
|
| mtub | gi|15608151 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase [Mycobacterium tuberculosis H37Rv] | 280 | 6.4e-77
|
| cglu | gi|19552137 COG1947:4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate synthase [Corynebacterium glutamicum] | 204 | 3.1e-54
|
| bsub | BG10109 ispE, yabH; 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase [EC:2.7.1.-] | 142 | 3.1e-35
|
| ecoli | gi|16129171 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase [Escherichia coli K12] | 77 | 1.1e-15
|
| paer | PA4669 [gene=ipk] [prot=isopentenyl monophosphate kinase] [comment=PA4669] | 84 | 9.1e-18
|
*coel, Streptomyces coelicolor A3(2); sgr, Streptomyces griseus IFO13350; sam, Streptomyces ambofaciens ATCC 23877; scab, Streptomyces scabies 87.22; sace, Saccharopolyspora erythraea NRRL2338; nfar, Nocardia farcinica IFM 10152; mtub, Mycobacterium tuberculosis H37Rv; cglu, Corynebacterium glutamicum; bsub, Bacillus subtilis; ecoli, Escherichia coli K-12; paer, Pseudomonas aeruginosa;
Pfam search result
| Model | No. | Description | Position (aa) | Score | E-value
|
| GHMP_kinases_C | 1 | GHMP kinases C terminal | 203-277 | 41.9 | 8.7e-11
|
| GHMP_kinases_N | 1 | GHMP kinases N terminal domain | 87-145 | 25.8 | 8.7e-06
|
BLAST search result (top 10 hits)
| ID | Description | Score | E-value
|
| NGO10876.1 | 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase [Strepto...
| 985 | 1.1e-131 |
| NGO48613.1 | 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase [Strepto...
| 963 | 1.5e-128 |
| MPY46705.1 | 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase [Strepto...
| 961 | 3.7e-128 |
| WP_151484375.1 | 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase [St...
| 958 | 9.8e-128 |
| WP_189186435.1 | 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase [St...
| 955 | 3.4e-127 |
| MPY48932.1 | 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase [Strepto...
| 954 | 4.5e-127 |
| WP_189899654.1 | 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase [St...
| 951 | 1.2e-126 |
| WP_071364047.1 | 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase [St...
| 951 | 1.4e-126 |
| WP_217452624.1 | 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase [St...
| 949 | 2.1e-126 |
| WP_059130690.1 | 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase [St...
| 950 | 2.9e-126 |
PSORT result
| Localization | Certainty | PSORT said
|
| Not Clear | | Seems to have no N-terminal signal seq.
|