| ID | SAVERM_3969
| Type | CDS
|
| From | 4895743
| nt length | 753
|
| To | 4894991
| aa length | 250
|
| Gene | ispD
| pI | 5.14
|
| Definition | putative 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
| M.W (Da) | 26033
|
| Note | MEP pathway, EC 2.7.7.60
| AseI frg. | G1
|
| SignalP v.4.0 | Non-secretory protein | plot
| Category | metabolism of coenzymes & prosthetic groups
|
Orthologs (if any)
| Bacteria* | Reciprocally best hit to | Score | E-value
|
| coel | SCO4233 hypothetical protein | 423 | 1.0e-152
|
| sgr | SGR_4012 putative 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase | 378 | 8.0e-135
|
| sam | SAM23877_3467 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase | 425 | 1.0e-153
|
| scab | SCAB50091 putative 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase | 426 | 8.0e-154
|
| sace | SACE_0439 4-diphosphocytidyl-2C-methyl-D-erythritol synthase | 170 | 2.0e-53
|
| nfar | nfa4360 mect 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase | 167 | 6.2e-43
|
| mtub | gi|15610718 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase [Mycobacterium tuberculosis H37Rv] | 171 | 5.6e-44
|
| cglu | gi|19553857 COG1211:4-diphosphocytidyl-2-methyl-D-erithritol synthase [Corynebacterium glutamicum] | 152 | 1.5e-38
|
| bsub | BG10152 ispD, yacM; 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase [EC:2.7.7.-] | 122 | 2.0e-29
|
| ecoli | gi|16130654 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase [Escherichia coli K12] | 102 | 3.2e-23
|
| paer | PA3633 [gene=PA3633] [prot=conserved hypothetical protein] [comment=PA3633] | 56 | 2.1e-09
|
*coel, Streptomyces coelicolor A3(2); sgr, Streptomyces griseus IFO13350; sam, Streptomyces ambofaciens ATCC 23877; scab, Streptomyces scabies 87.22; sace, Saccharopolyspora erythraea NRRL2338; nfar, Nocardia farcinica IFM 10152; mtub, Mycobacterium tuberculosis H37Rv; cglu, Corynebacterium glutamicum; bsub, Bacillus subtilis; ecoli, Escherichia coli K-12; paer, Pseudomonas aeruginosa;
Pfam search result
| Model | No. | Description | Position (aa) | Score | E-value
|
| IspD | 1 | 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase | 15-242 | 224.3 | 1.4e-66
|
| NTP_transf_3 | 1 | MobA-like NTP transferase domain | 16-147 | 49.7 | 4.8e-13
|
BLAST search result (top 10 hits)
| ID | Description | Score | E-value
|
| WP_189836787.1 | 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase [St...
| 932 | 3.0e-125 |
| WP_217454028.1 | 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase [St...
| 928 | 1.3e-124 |
| WP_200396726.1 | 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase [St...
| 924 | 4.8e-124 |
| WP_151485017.1 | 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase [St...
| 924 | 5.8e-124 |
| WP_143639567.1 | 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase [St...
| 921 | 1.2e-123 |
| WP_189186134.1 | 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase [St...
| 918 | 3.7e-123 |
| WP_235477188.1 | 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase [St...
| 917 | 5.2e-123 |
| WP_105974770.1 | 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase [St...
| 913 | 2.5e-122 |
| WP_189145196.1 | 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase [St...
| 912 | 3.0e-122 |
| WP_164317605.1 | 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase [St...
| 910 | 6.1e-122 |
PSORT result
| Localization | Certainty | PSORT said
|
| membrane | 0.1510 | Seems to have no N-terminal signal seq.
|