| ID | SAVERM_4115
| Type | CDS
|
| From | 5046990
| nt length | 873
|
| To | 5047862
| aa length | 290
|
| Gene | trmB
| pI | 6.68
|
| Definition | putative tRNA (guanine-N(7)-)-methyltransferase
| M.W (Da) | 32245
|
| Note | PF02390: Putative methyltransferase
| AseI frg. | H
|
| SignalP v.4.0 | Non-secretory protein | plot
| Category | RNA modification
|
Orthologs (if any)
| Bacteria* | Reciprocally best hit to | Score | E-value
|
| coel | SCO4111 conserved hypothetical protein | 451 | 1.0e-162
|
| sgr | SGR_3900 putative methyltransferase | 398 | 2.0e-141
|
| sam | SAM23877_3574 tRNA (guanine-N(7)-)-methyltransferase | 438 | 5.0e-157
|
| scab | SCAB49051 putative methyltransferase | 444 | 1.0e-159
|
| sace | SACE_7256 tRNA (guanine-N7-)-methyltransferase | 224 | 1.0e-73
|
| nfar | nfa54840 methyltransferase | 184 | 7.9e-48
|
| mtub | gi|15607349 tRNA (guanine-N(7)-)-methyltransferase [Mycobacterium tuberculosis H37Rv] | 157 | 6.0e-40
|
| cglu | gi|19554055 COG0220:Predicted S-adenosylmethionine-dependent methyltransferase [Corynebacterium glutamicum] | 160 | 8.6e-41
|
| bsub | BG13890 ytmQ; hypothetical methlytransferase [EC:2.1.1.-] | 70 | 1.4e-13
|
| ecoli | gi|16130861 tRNA (guanine-N(7)-)-methyltransferase [Escherichia coli K12] | 211 | 6.0e-56
|
| paer | PA0382 [gene=micA] [prot=DNA mismatch repair protein MicA] [comment=PA0382] | 216 | 2.5e-57
|
*coel, Streptomyces coelicolor A3(2); sgr, Streptomyces griseus IFO13350; sam, Streptomyces ambofaciens ATCC 23877; scab, Streptomyces scabies 87.22; sace, Saccharopolyspora erythraea NRRL2338; nfar, Nocardia farcinica IFM 10152; mtub, Mycobacterium tuberculosis H37Rv; cglu, Corynebacterium glutamicum; bsub, Bacillus subtilis; ecoli, Escherichia coli K-12; paer, Pseudomonas aeruginosa;
Pfam search result
| Model | No. | Description | Position (aa) | Score | E-value
|
| Methyltransf_4 | 1 | Putative methyltransferase | 99-265 | 174.9 | 9.4e-52
|
BLAST search result (top 10 hits)
| ID | Description | Score | E-value
|
| WP_189711348.1 | tRNA (guanosine(46)-N7)-methyltransferase TrmB [Streptomyces...
| 1185 | 3.2e-162 |
| WP_190154307.1 | tRNA (guanosine(46)-N7)-methyltransferase TrmB [Streptomyces...
| 1181 | 4.5e-162 |
| GGY15186.1 | tRNA (guanine-N(7)-)-methyltransferase [Streptomyces anandii JCM...
| 1181 | 3.6e-161 |
| WP_220647875.1 | tRNA (guanosine(46)-N7)-methyltransferase TrmB [Streptomyces...
| 1176 | 4.4e-161 |
| WP_230217182.1 | tRNA (guanosine(46)-N7)-methyltransferase TrmB [Streptomyces...
| 1169 | 5.1e-160 |
| WP_234379518.1 | tRNA (guanosine(46)-N7)-methyltransferase TrmB [Streptomyces...
| 1167 | 1.2e-159 |
| WP_229864997.1 | tRNA (guanosine(46)-N7)-methyltransferase TrmB [Streptomyces...
| 1167 | 1.4e-159 |
|
| 1166 | 1.4e-159 |
| WP_173877722.1 | tRNA (guanosine(46)-N7)-methyltransferase TrmB [Streptomyces...
| 1165 | 1.5e-159 |
| WP_234442855.1 | MULTISPECIES: tRNA (guanosine(46)-N7)-methyltransferase TrmB...
| 1164 | 3.1e-159 |
PSORT result
| Localization | Certainty | PSORT said
|
| cytoplasm | 0.4390 | Seems to have no N-terminal signal seq.
|