| ID | SAVERM_4364
| Type | CDS
|
| From | 5341986
| nt length | 1368
|
| To | 5343353
| aa length | 455
|
| Gene | bkdC
| pI | 5.74
|
| Definition | putative dihydrolipoamide acyltransferase
| M.W (Da) | 47116
|
| Note | PF00198: 2-oxoacid dehydrogenases acyltransferase (catalytic domain)
| AseI frg. | H
|
| SignalP v.4.0 | Non-secretory protein | plot
| Category | metabolism of lipids
|
Orthologs (if any)
| Bacteria* | Reciprocally best hit to | Score | E-value
|
| coel | SCO3829 putative dihydrolipoamide acyltransferase component E2 | 647 | 0.0e+00
|
| sgr | SGR_3750 putative dihydrolipoamide acyltransferase component E2 | 600 | 0.0e+00
|
| sam | SAM23877_3828 putative dihydrolipoamide acyltransferase component E2 | 645 | 0.0e+00
|
| scab | SCAB45291 putative dihydrolipoamide acyltransferase | 662 | 0.0e+00
|
| sace | SACE_5544 putative dihydrolipoamide acyltransferase component E2 | 477 | 4.0e-168
|
| mtub | gi|15609632 dihydrolipoamide acetyltransferase [Mycobacterium tuberculosis H37Rv] | 249 | 3.5e-67
|
| paer | PA2249 [gene=bkdB] [prot=branched-chain alpha-keto acid dehydrogenase (lipoamide component)] [comment=PA2249] | 256 | 3.9e-69
|
*coel, Streptomyces coelicolor A3(2); sgr, Streptomyces griseus IFO13350; sam, Streptomyces ambofaciens ATCC 23877; scab, Streptomyces scabies 87.22; sace, Saccharopolyspora erythraea NRRL2338; mtub, Mycobacterium tuberculosis H37Rv; paer, Pseudomonas aeruginosa;
Pfam search result
| Model | No. | Description | Position (aa) | Score | E-value
|
| 2-oxoacid_dh | 1 | 2-oxoacid dehydrogenases acyltransferase (catalytic domain) | 228-452 | 239.8 | 2.4e-71
|
| Biotin_lipoyl | 1 | Biotin-requiring enzyme | 6-77 | 60.9 | 7e-17
|
| E3_binding | 1 | e3 binding domain | 163-197 | 53.7 | 1.8e-14
|
BLAST search result (top 10 hits)
| ID | Description | Score | E-value
|
| WP_232651055.1 | 2-oxo acid dehydrogenase subunit E2 [Streptomyces sp. NR30] ...
| 1545 | 0.0e+00 |
| WP_037674971.1 | 2-oxo acid dehydrogenase subunit E2 [Streptomyces griseus]
| 1535 | 0.0e+00 |
|
| 1532 | 0.0e+00 |
| WP_095751652.1 | 2-oxo acid dehydrogenase subunit E2 [Streptomyces sp. SA15] ...
| 1530 | 0.0e+00 |
| WP_046256572.1 | 2-oxo acid dehydrogenase subunit E2 [Streptomyces sp. WM6386...
| 1527 | 0.0e+00 |
| WP_046703116.1 | 2-oxo acid dehydrogenase subunit E2 [Streptomyces europaeisc...
| 1527 | 0.0e+00 |
| WP_045558552.1 | MULTISPECIES: 2-oxo acid dehydrogenase subunit E2 [Streptomy...
| 1526 | 0.0e+00 |
| WP_067436264.1 | 2-oxo acid dehydrogenase subunit E2 [Streptomyces lincolnens...
| 1523 | 0.0e+00 |
| WP_030939121.1 | 2-oxo acid dehydrogenase subunit E2 [Streptomyces sp. NRRL S...
| 1522 | 0.0e+00 |
| WP_123966270.1 | 2-oxo acid dehydrogenase subunit E2 [Streptomyces sp. TLI_18...
| 1521 | 0.0e+00 |
PSORT result
| Localization | Certainty | PSORT said
|
| membrane | 0.1723 | Seems to have no N-terminal signal seq.
|