| ID | SAVERM_4664
| Type | CDS
|
| From | 5689055
| nt length | 1038
|
| To | 5690092
| aa length | 345
|
| Gene | tilS
| pI | 9.29
|
| Definition | putative tRNA(Ile)-lysidine synthase
| M.W (Da) | 36173
|
| Note | PF01171: PP-loop family
| AseI frg. | R
|
| SignalP v.4.0 | Non-secretory protein | plot
| Category | cell division
|
Orthologs (if any)
| Bacteria* | Reciprocally best hit to | Score | E-value
|
| coel | SCO3406 hypothetical protein | 509 | 0.0e+00
|
| sgr | SGR_4086 conserved hypothetical protein | 471 | 5.0e-168
|
| sam | SAM23877_4157 tRNA(Ile)-lysidine synthase | 513 | 0.0e+00
|
| scab | SCAB39801 putative tRNA(Ile)-lysidine synthase | 545 | 0.0e+00
|
| sace | SACE_0394 cell cycle protein MesJ | 196 | 6.0e-61
|
| nfar | nfa3880 hypothetical protein | 226 | 2.9e-60
|
| mtub | gi|15610761 POSSIBLE CELL CYCLE PROTEIN MESJ [Mycobacterium tuberculosis H37Rv] | 207 | 6.3e-55
|
| cglu | gi|19553892 COG0037:Predicted ATPase of the PP-loop superfamily implicated in cell cycle control [Corynebacterium glutamicum] | 117 | 7.9e-28
|
| bsub | BG10130 yacA; unknown | 86 | 1.9e-18
|
| ecoli | gi|16128181 tRNA(Ile)-lysidine synthetase [Escherichia coli K12] | 87 | 1.2e-18
|
| paer | PA3638 [gene=PA3638] [prot=conserved hypothetical protein] [comment=PA3638] | 90 | 2.0e-19
|
*coel, Streptomyces coelicolor A3(2); sgr, Streptomyces griseus IFO13350; sam, Streptomyces ambofaciens ATCC 23877; scab, Streptomyces scabies 87.22; sace, Saccharopolyspora erythraea NRRL2338; nfar, Nocardia farcinica IFM 10152; mtub, Mycobacterium tuberculosis H37Rv; cglu, Corynebacterium glutamicum; bsub, Bacillus subtilis; ecoli, Escherichia coli K-12; paer, Pseudomonas aeruginosa;
Pfam search result
| Model | No. | Description | Position (aa) | Score | E-value
|
| ATP_bind_3 | 1 | PP-loop family | 46-224 | 131.4 | 2.7e-38
|
| TilS | 1 | TilS substrate binding domain | 273-340 | 43.4 | 3.4e-11
|
BLAST search result (top 10 hits)
| ID | Description | Score | E-value
|
| WP_095853311.1 | MULTISPECIES: tRNA lysidine(34) synthetase TilS [unclassifie...
| 1220 | 3.7e-166 |
| WP_184975296.1 | tRNA lysidine(34) synthetase TilS [Streptomyces echinatus] >...
| 1206 | 8.0e-164 |
| WP_019064279.1 | tRNA lysidine(34) synthetase TilS [Streptomyces prunicolor]
| 1206 | 8.1e-164 |
| WP_031165036.1 | tRNA lysidine(34) synthetase TilS [Streptomyces durhamensis]
| 1204 | 1.6e-163 |
| WP_119101438.1 | tRNA lysidine(34) synthetase TilS [Streptomyces sporangiifor...
| 1204 | 2.9e-163 |
| WP_043685312.1 | tRNA lysidine(34) synthetase TilS [Streptomyces xylophagus]
| 1202 | 2.9e-163 |
| WP_184909448.1 | tRNA lysidine(34) synthetase TilS [Streptomyces luteogriseus...
| 1202 | 3.1e-163 |
| WP_191875761.1 | tRNA lysidine(34) synthetase TilS [Streptomyces filipinensis...
| 1201 | 4.5e-163 |
| WP_200688276.1 | tRNA lysidine(34) synthetase TilS [Streptomyces sp. MBT65] >...
| 1201 | 4.6e-163 |
| WP_019070221.1 | MULTISPECIES: tRNA lysidine(34) synthetase TilS [Streptomyce...
| 1201 | 4.7e-163 |
PSORT result
| Localization | Certainty | PSORT said
|
| cytoplasm | 0.0814 | Seems to have no N-terminal signal seq.
|