| ID | SAVERM_5427
| Type | CDS
|
| From | 6580410
| nt length | 810
|
| To | 6581219
| aa length | 269
|
| Gene | nei2
| pI | 7.96
|
| Definition | putative endonuclease VIII and DNA N-glycosylase with an AP lyase activity
| M.W (Da) | 29714
|
| Note | involved in DNA repair; nick DNA at apurinic/apyrimidinic sites. PF01149: Formamidopyrimidine-DNA glycosylase N-terminal domain
| AseI frg. | B
|
| SignalP v.4.0 | Non-secretory protein | plot
| Category | DNA modification & repair
|
Orthologs (if any)
| Bacteria* | Reciprocally best hit to | Score | E-value
|
| coel | SCO2626 putative DNA repair hydrolase (fragment) | 461 | 6.0e-167
|
| sgr | SGR_4916 putative DNA repair hydrolase | 424 | 2.0e-152
|
| sam | SAM23877_2676 Endonuclease 8 1 | 466 | 5.0e-169
|
| scab | SCAB59861 putative endonuclease VIII and DNA N-glycosylase with an AP lyase activity | 427 | 1.0e-153
|
| sace | SACE_1351 formamidopyrimidine-DNA glycosylase | 345 | 2.0e-121
|
| nfar | nfa13280 formamidopyrimidine-DNA glycosylase | 294 | 4.8e-81
|
| mtub | gi|15609601 POSSIBLE DNA GLYCOSYLASE [Mycobacterium tuberculosis H37Rv] | 273 | 6.8e-75
|
| cglu | gi|19554194 COG0266:Formamidopyrimidine-DNA glycosylase [Corynebacterium glutamicum] | 279 | 1.1e-76
|
*coel, Streptomyces coelicolor A3(2); sgr, Streptomyces griseus IFO13350; sam, Streptomyces ambofaciens ATCC 23877; scab, Streptomyces scabies 87.22; sace, Saccharopolyspora erythraea NRRL2338; nfar, Nocardia farcinica IFM 10152; mtub, Mycobacterium tuberculosis H37Rv; cglu, Corynebacterium glutamicum;
Pfam search result
| Model | No. | Description | Position (aa) | Score | E-value
|
| H2TH | 1 | Formamidopyrimidine-DNA glycosylase H2TH domain | 122-205 | 76.5 | 1.2e-21
|
| Fapy_DNA_glyco | 1 | Formamidopyrimidine-DNA glycosylase N-terminal domain | 1-103 | 37.9 | 2.1e-09
|
| zf-FPG_IleRS | 1 | Zinc finger found in FPG and IleRS | 242-268 | 34.4 | 1.3e-08
|
BLAST search result (top 10 hits)
| ID | Description | Score | E-value
|
| WP_202498418.1 | Fpg/Nei family DNA glycosylase [Streptomyces sp. SID5469]
| 1231 | 2.2e-170 |
| WP_099922656.1 | Fpg/Nei family DNA glycosylase [Streptomyces sp. GbtcB7]
| 1177 | 6.3e-162 |
| WP_075031823.1 | Fpg/Nei family DNA glycosylase [Streptomyces mirabilis] >emb...
| 1174 | 2.0e-161 |
| WP_190168881.1 | Fpg/Nei family DNA glycosylase [Streptomyces mirabilis] >dbj...
| 1170 | 6.3e-161 |
| WP_060897743.1 | MULTISPECIES: Fpg/Nei family DNA glycosylase [unclassified S...
| 1165 | 4.4e-160 |
| WP_097284725.1 | Fpg/Nei family DNA glycosylase [Streptomyces sp. OK228] >emb...
| 1158 | 5.5e-159 |
| WP_168491528.1 | MULTISPECIES: Fpg/Nei family DNA glycosylase [Streptomyces] ...
| 1157 | 6.3e-159 |
| QIY95924.1 | Fpg/Nei family DNA glycosylase [Streptomyces sp. S1D4-11]
| 1156 | 1.1e-158 |
| WP_095854680.1 | MULTISPECIES: Fpg/Nei family DNA glycosylase [unclassified S...
| 1155 | 1.4e-158 |
| WP_067370188.1 | Fpg/Nei family DNA glycosylase [Streptomyces olivochromogene...
| 1154 | 1.9e-158 |
PSORT result
| Localization | Certainty | PSORT said
|
| cytoplasm | 0.1713 | Seems to have no N-terminal signal seq.
|