| ID | SAVERM_5491
| Type | CDS
|
| From | 6666768
| nt length | 1164
|
| To | 6667931
| aa length | 387
|
| Gene | comEA
| pI | 10.9
|
| Definition | putative exogenous DNA-binding protein
| M.W (Da) | 39122
|
| Note | PF00633: Helix-hairpin-helix motif
| AseI frg. | B
|
| SignalP v.4.0 | Non-secretory protein | plot
| Category | regulation
|
Orthologs (if any)
| Bacteria* | Reciprocally best hit to | Score | E-value
|
| coel | SCO2568 putative DNA-binding protein | 378 | 3.0e-131
|
| sgr | SGR_4976 putative DNA-binding protein | 221 | 7.0e-70
|
| sam | SAM23877_2615 putative DNA-binding protein | 318 | 1.0e-107
|
| scab | SCAB60391 putative exogenous DNA-binding protein | 289 | 5.0e-97
|
| sace | SACE_1435 competence protein ComEA helix-hairpin-helix region | 126 | 3.0e-35
|
| nfar | nfa13820 DNA-binding protein | 144 | 1.7e-35
|
| mtub | gi|15609552 hypothetical protein Rv2415c [Mycobacterium tuberculosis H37Rv] | 130 | 1.9e-31
|
| cglu | gi|19553547 COG1555:DNA uptake protein and related DNA-binding proteins [Corynebacterium glutamicum] | 106 | 1.6e-24
|
| bsub | BG10480 comEA, comE1, comD; exogenous DNA-binding protein | 89 | 2.6e-19
|
| ecoli | gi|16128427 hypothetical protein b0442 [Escherichia coli K12] | 48 | 9.6e-07
|
| paer | PA3140 [gene=PA3140] [prot=hypothetical protein] [comment=PA3140] | 48 | 1.0e-06
|
*coel, Streptomyces coelicolor A3(2); sgr, Streptomyces griseus IFO13350; sam, Streptomyces ambofaciens ATCC 23877; scab, Streptomyces scabies 87.22; sace, Saccharopolyspora erythraea NRRL2338; nfar, Nocardia farcinica IFM 10152; mtub, Mycobacterium tuberculosis H37Rv; cglu, Corynebacterium glutamicum; bsub, Bacillus subtilis; ecoli, Escherichia coli K-12; paer, Pseudomonas aeruginosa;
Pfam search result
| Model | No. | Description | Position (aa) | Score | E-value
|
| HHH_3 | 1 | Helix-hairpin-helix motif | 325-385 | 76.1 | 1.7e-21
|
| SLBB | 1 | SLBB domain | 244-297 | 39.0 | 5.1e-10
|
| HHH | 1 | Helix-hairpin-helix motif | 328-354 | 37.1 | 1.5e-09
|
BLAST search result (top 10 hits)
| ID | Description | Score | E-value
|
| WP_075032715.1 | ComEA family DNA-binding protein [Streptomyces mirabilis]
| 823 | 2.4e-104 |
| WP_217565657.1 | ComEA family DNA-binding protein [Streptomyces sp. GbtcB7]
| 787 | 8.0e-99 |
| WP_151481537.1 | ComEA family DNA-binding protein [Streptomyces albicerus]
| 781 | 8.5e-99 |
| WP_097284690.1 | ComEA family DNA-binding protein [Streptomyces sp. OK228]
| 787 | 8.6e-99 |
| WP_143608051.1 | MULTISPECIES: ComEA family DNA-binding protein [unclassified...
| 783 | 3.8e-98 |
| WP_143601608.1 | MULTISPECIES: ComEA family DNA-binding protein [unclassified...
| 779 | 1.3e-97 |
| WP_190168834.1 | ComEA family DNA-binding protein [Streptomyces mirabilis]
| 775 | 5.1e-97 |
| WP_143613362.1 | MULTISPECIES: ComEA family DNA-binding protein [unclassified...
| 773 | 1.5e-96 |
| WP_103838321.1 | ComEA family DNA-binding protein [Streptomyces sp. Ru72] >gb...
| 767 | 2.5e-96 |
| WP_079065073.1 | ComEA family DNA-binding protein [Streptomyces olivochromoge...
| 765 | 2.0e-95 |
PSORT result
| Localization | Certainty | PSORT said
|
| membrane | 0.4736 | Seems to have no N-terminal signal seq.
|