| ID | SAVERM_5649
| Type | CDS
|
| From | 6829246
| nt length | 1206
|
| To | 6830451
| aa length | 401
|
| Gene | dus
| pI | 6.19
|
| Definition | putative dihydrouridine synthase
| M.W (Da) | 42927
|
| Note | PF01207: Dihydrouridine synthase (Dus)
| AseI frg. | B
|
| SignalP v.4.0 | Non-secretory protein | plot
| Category | metabolism of nucleotides & nucleic acids
|
Orthologs (if any)
| Bacteria* | Reciprocally best hit to | Score | E-value
|
| coel | SCO2497 hypothetical protein | 673 | 0.0e+00
|
| sgr | SGR_5047 putative dihydrouridine synthase | 661 | 0.0e+00
|
| sam | SAM23877_2547 putative tRNA-dihydrouridine synthase | 704 | 0.0e+00
|
| scab | SCAB62091 putative dihydrouridine synthase | 708 | 0.0e+00
|
| sace | SACE_7086 dihydrouridine synthase TIM-barrel protein nifR3 | 462 | 1.0e-163
|
| nfar | nfa6060 dihydrouridine synthase | 424 | 6.8e-120
|
| mtub | gi|15607963 POSSIBLE TRANSCRIPTIONAL REGULATORY PROTEIN [Mycobacterium tuberculosis H37Rv] | 402 | 2.8e-113
|
| cglu | gi|19553766 COG0042:Predicted TIM-barrel enzymes, possibly dehydrogenases, nifR3 family [Corynebacterium glutamicum] | 381 | 3.5e-107
|
| bsub | BG10143 yacF; similar to transcriptional regulator (nitrogen regulation protein) | 202 | 2.1e-53
|
| ecoli | gi|16131148 conserved protein, FMN-linked [Escherichia coli K12] | 171 | 6.0e-44
|
| paer | PA4852 [gene=PA4852] [prot=conserved hypothetical protein] [comment=PA4852] | 196 | 3.1e-51
|
*coel, Streptomyces coelicolor A3(2); sgr, Streptomyces griseus IFO13350; sam, Streptomyces ambofaciens ATCC 23877; scab, Streptomyces scabies 87.22; sace, Saccharopolyspora erythraea NRRL2338; nfar, Nocardia farcinica IFM 10152; mtub, Mycobacterium tuberculosis H37Rv; cglu, Corynebacterium glutamicum; bsub, Bacillus subtilis; ecoli, Escherichia coli K-12; paer, Pseudomonas aeruginosa;
Pfam search result
| Model | No. | Description | Position (aa) | Score | E-value
|
| Dus | 1 | Dihydrouridine synthase (Dus) | 24-344 | 308.1 | 5.6e-92
|
BLAST search result (top 10 hits)
| ID | Description | Score | E-value
|
| WP_189185211.1 | tRNA dihydrouridine synthase DusB [Streptomyces albiflavesce...
| 1871 | 0.0e+00 |
| GHD42104.1 | tRNA dihydrouridine synthase DusB [Streptomyces mirabilis]
| 1867 | 0.0e+00 |
| GAX50022.1 | tRNA dihydrouridine synthase DusB [Streptomyces olivochromogenes...
| 1867 | 0.0e+00 |
| WP_105971810.1 | tRNA dihydrouridine synthase DusB [Streptomyces geranii]
| 1866 | 0.0e+00 |
| WP_190168779.1 | tRNA dihydrouridine synthase DusB [Streptomyces mirabilis]
| 1865 | 0.0e+00 |
| WP_067370563.1 | tRNA dihydrouridine synthase DusB [Streptomyces olivochromog...
| 1864 | 0.0e+00 |
| WP_143601580.1 | MULTISPECIES: tRNA dihydrouridine synthase DusB [unclassifie...
| 1863 | 0.0e+00 |
| WP_168491303.1 | MULTISPECIES: tRNA dihydrouridine synthase DusB [Streptomyce...
| 1861 | 0.0e+00 |
| PBC99186.1 | nifR3 family TIM-barrel protein [Streptomyces sp. Ag82_O1-15] >em...
| 1858 | 0.0e+00 |
| SOE27574.1 | putative TIM-barrel protein, nifR3 family [Streptomyces sp. OK22...
| 1858 | 0.0e+00 |
PSORT result
| Localization | Certainty | PSORT said
|
| cytoplasm | 0.0988 | Seems to have no N-terminal signal seq.
|