| ID | SAVERM_5676
| Type | CDS
|
| From | 6864460
| nt length | 1899
|
| To | 6866358
| aa length | 632
|
| Gene | dnaG
| pI | 6.18
|
| Definition | putative DNA primase
| M.W (Da) | 69200
|
| Note | PF01807: CHC2 zinc finger
| AseI frg. | B
|
| SignalP v.4.0 | Non-secretory protein | plot
| Category | DNA replication
|
Orthologs (if any)
| Bacteria* | Reciprocally best hit to | Score | E-value
|
| coel | SCO2468 DNA primase | 1130 | 0.0e+00
|
| sgr | SGR_5066 putative DNA primase | 978 | 0.0e+00
|
| sam | SAM23877_2521 DNA primase | 1116 | 0.0e+00
|
| scab | SCAB62491 putative DNA primase | 1143 | 0.0e+00
|
| sace | SACE_1506 DNA primase (bacterial type) | 588 | 0.0e+00
|
| nfar | nfa14700 dnaG DNA primase | 580 | 1.3e-166
|
| mtub | gi|15609480 DNA primase [Mycobacterium tuberculosis H37Rv] | 549 | 1.8e-157
|
| cglu | gi|19553466 COG0358:DNA primase (bacterial type) [Corynebacterium glutamicum] | 543 | 7.0e-156
|
| bsub | BG10313 dnaG, dnaE; DNA primase [EC:2.7.7.-] | 250 | 2.0e-67
|
| ecoli | gi|16130962 DNA primase [Escherichia coli K12] | 251 | 7.7e-68
|
| paer | PA0577 [gene=dnaG] [prot=DNA primase] [comment=PA0577] | 251 | 1.1e-67
|
*coel, Streptomyces coelicolor A3(2); sgr, Streptomyces griseus IFO13350; sam, Streptomyces ambofaciens ATCC 23877; scab, Streptomyces scabies 87.22; sace, Saccharopolyspora erythraea NRRL2338; nfar, Nocardia farcinica IFM 10152; mtub, Mycobacterium tuberculosis H37Rv; cglu, Corynebacterium glutamicum; bsub, Bacillus subtilis; ecoli, Escherichia coli K-12; paer, Pseudomonas aeruginosa;
Pfam search result
| Model | No. | Description | Position (aa) | Score | E-value
|
| Toprim_N | 1 | DNA primase catalytic core, N-terminal domain | 127-254 | 123.0 | 6.9e-36
|
| zf-CHC2 | 1 | CHC2 zinc finger | 6-101 | 109.8 | 4.1e-32
|
| Toprim_4 | 1 | Toprim domain | 263-333 | 62.6 | 3.5e-17
|
| DnaG_DnaB_bind | 1 | DNA primase DnaG DnaB-binding | 483-599 | 54.7 | 1.4e-14
|
| DnaB_bind | 1 | DnaB-helicase binding domain of primase | 374-427 | 51.9 | 5.8e-14
|
| Toprim_2 | 1 | Toprim-like | 265-353 | 41.2 | 1.6e-10
|
| Toprim | 1 | Toprim domain | 264-331 | 28.2 | 1.6e-06
|
BLAST search result (top 10 hits)
PSORT result
| Localization | Certainty | PSORT said
|
| cytoplasm | 0.1484 | Seems to have no N-terminal signal seq.
|