| ID | SAVERM_5948
| Type | CDS
|
| From | 7162291
| nt length | 1755
|
| To | 7160537
| aa length | 584
|
| Gene | nadE
| pI | 4.77
|
| Definition | putative NH(3)-dependent NAD(+) synthetase
| M.W (Da) | 63044
|
| Note | PF02540: NAD synthase
| AseI frg. | F
|
| SignalP v.4.0 | Non-secretory protein | plot
| Category | metabolism of coenzymes & prosthetic groups
|
Orthologs (if any)
| Bacteria* | Reciprocally best hit to | Score | E-value
|
| coel | SCO2238 nadE NAD(+) synthase (glutamine-hydrolysing) | 1021 | 0.0e+00
|
| sgr | SGR_5258 putative NH(3)-dependent NAD(+) synthetase | 1032 | 0.0e+00
|
| sam | SAM23877_2326 Glutamine-dependent NAD(+) synthetase | 982 | 0.0e+00
|
| scab | SCAB66451 putative NH(3)-dependent NAD(+) synthetase | 1078 | 0.0e+00
|
| sace | SACE_1610 glutamine-dependent NAD(+) synthetase | 682 | 0.0e+00
|
| mtub | gi|57116990 NAD(+) synthetase [Mycobacterium tuberculosis H37Rv] | 165 | 6.7e-42
|
| bsub | BG10694 nadE, outB, tscBGH, gsp-81; NH3-dependent NAD+ synthetase [EC:6.3.5.1] | 55 | 1.1e-08
|
| ecoli | gi|16130706 L-1,2-propanediol oxidoreductase [Escherichia coli K12] | 30 | 4.4e-01
|
*coel, Streptomyces coelicolor A3(2); sgr, Streptomyces griseus IFO13350; sam, Streptomyces ambofaciens ATCC 23877; scab, Streptomyces scabies 87.22; sace, Saccharopolyspora erythraea NRRL2338; mtub, Mycobacterium tuberculosis H37Rv; bsub, Bacillus subtilis; ecoli, Escherichia coli K-12;
Pfam search result
| Model | No. | Description | Position (aa) | Score | E-value
|
| NAD_synthase | 1 | NAD synthase | 309-556 | 222.4 | 4.3e-66
|
| CN_hydrolase | 1 | Carbon-nitrogen hydrolase | 5-262 | 86.8 | 1.4e-24
|
BLAST search result (top 10 hits)
PSORT result
| Localization | Certainty | PSORT said
|
| membrane | 0.2168 | Seems to have no N-terminal signal seq.
|