| ID | SAVERM_5982 | Type | CDS |
| From | 7202286 | nt length | 5421 |
| To | 7207706 | aa length | 1806 |
| Gene | amyX | pI | 6.18 |
| Definition | putative pullulanase, secreted | M.W (Da) | 194324 |
| Note | PF00128: Alpha amylase, catalytic domain | AseI frg. | F |
| SignalP v.4.0 | Signal peptide | plot | Category | specific pathways |
| Bacteria* | Reciprocally best hit to | Score | E-value |
| coel | SCO2226 putative bi-functional protein (secreted alpha-amylase/dextrinase) | 2923 | 0.0e+00 |
| sgr | SGR_5281 putative alpha-amylase | 2493 | 0.0e+00 |
| sam | SAM23877_2312 Bi-functional protein (Secreted alpha-amylase/dextrinase) | 2882 | 0.0e+00 |
| scab | SCAB66701 putative pullulanase, secreted | 2984 | 0.0e+00 |
| bsub | BG12566 amyX; pullulanase [EC:3.2.1.41] | 216 | 1.3e-56 |
| ecoli | gi|16131442 periplasmic alpha-amylase precursor [Escherichia coli K12] | 148 | 3.7e-36 |
| Model | No. | Description | Position (aa) | Score | E-value | |
| Alpha-amylase | 1 | Alpha amylase, catalytic domain | 108-478 | 356.4 | 1.8e-106 | |
| PUD | 2 | Bacterial pullanase-associated domain | 738-833, 847-937 | 165.5 | 3.6e-49 | |
| DUF3372 | 1 | Domain of unknown function (DUF3372) | 1654-1803 | 149.3 | 6.2e-44 | |
| CBM_48 | 1 | Carbohydrate-binding module 48 (Isoamylase N-terminal domain) | 1077-1155 | 39.0 | 7.1e-10 | |
| Alpha-amylase_C | 1 | Alpha amylase, C-terminal all-beta domain | 549-622 | 26.3 | 7.3e-06 |
| ID | Description | Score | E-value |
| GGN84602.1 | 1,4-alpha-glucan branching enzyme [Streptomyces albiflavescens] | 8159 | 0.0e+00 |
| WP_189190620.1 | pullulanase-type alpha-1,6-glucosidase [Streptomyces albifla... | 8150 | 0.0e+00 |
| SFE75761.1 | alpha-1,6-glucosidases, pullulanase-type [Streptomyces mirabilis... | 8117 | 0.0e+00 |
| SOE27203.1 | alpha-1,6-glucosidases, pullulanase-type [Streptomyces sp. OK228... | 8115 | 0.0e+00 |
| WP_143657036.1 | pullulanase-type alpha-1,6-glucosidase [Streptomyces sp. S1A... | 8115 | 0.0e+00 |
| WP_143611160.1 | MULTISPECIES: pullulanase-type alpha-1,6-glucosidase [unclas... | 8112 | 0.0e+00 |
| WP_075026844.1 | pullulanase-type alpha-1,6-glucosidase [Streptomyces mirabil... | 8111 | 0.0e+00 |
| WP_143605366.1 | MULTISPECIES: pullulanase-type alpha-1,6-glucosidase [unclas... | 8110 | 0.0e+00 |
| WP_181652986.1 | pullulanase-type alpha-1,6-glucosidase [Streptomyces sp. WAC... | 8109 | 0.0e+00 |
| WP_097288904.1 | pullulanase-type alpha-1,6-glucosidase [Streptomyces sp. OK2... | 8108 | 0.0e+00 |
| Localization | Certainty | PSORT said |
| membrane | 0.1680 | Seems to have no N-terminal signal seq. |