| ID | SAVERM_6120
| Type | CDS
|
| From | 7360888
| nt length | 1404
|
| To | 7362291
| aa length | 467
|
| Gene | murD
| pI | 4.9
|
| Definition | putative UDP-N-acetylmuramoylalanine-D-glutamate ligase
| M.W (Da) | 48546
|
| Note | PF01225: Mur ligase family, catalytic domain
| AseI frg. | F
|
| SignalP v.4.0 | Non-secretory protein | plot
| Category | cell wall
|
Orthologs (if any)
| Bacteria* | Reciprocally best hit to | Score | E-value
|
| coel | SCO2086 putative UDP-N-acetylmuramoylalanine-D-glutamate ligase | 793 | 0.0e+00
|
| sgr | SGR_5419 putative UDP-N-acetylmuramoylalanine-D-glutamate ligase | 755 | 0.0e+00
|
| sam | SAM23877_2177 UDP-N-acetylmuramoylalanine--D-glutamate ligase | 783 | 0.0e+00
|
| scab | SCAB67991 putative UDP-N-acetylmuramoylalanine-D-glutamate ligase | 806 | 0.0e+00
|
| sace | SACE_5853 UDP-N-acetylmuramoylalanine--D-glutamate ligase | 387 | 6.0e-132
|
| nfar | nfa17640 murD UDP-N-acetylmuramoylalanine-D-glutamate ligase | 387 | 1.1e-108
|
| mtub | gi|15609292 UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase [Mycobacterium tuberculosis H37Rv] | 380 | 7.9e-107
|
| cglu | gi|19553360 COG0771:UDP-N-acetylmuramoylalanine-D-glutamate ligase [Corynebacterium glutamicum] | 325 | 3.6e-90
|
| bsub | BG10225 murD; UDP-N-acetylmuramoylalanine--D-glutamate ligase [EC:6.3.2.9] | 187 | 8.6e-49
|
| ecoli | gi|16128081 UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase [Escherichia coli K12] | 201 | 8.5e-53
|
| paer | PA4414 [gene=murD] [prot=UDP-N-acetylmuramoylalanine--D-glutamate ligase] [comment=PA4414] | 228 | 6.9e-61
|
*coel, Streptomyces coelicolor A3(2); sgr, Streptomyces griseus IFO13350; sam, Streptomyces ambofaciens ATCC 23877; scab, Streptomyces scabies 87.22; sace, Saccharopolyspora erythraea NRRL2338; nfar, Nocardia farcinica IFM 10152; mtub, Mycobacterium tuberculosis H37Rv; cglu, Corynebacterium glutamicum; bsub, Bacillus subtilis; ecoli, Escherichia coli K-12; paer, Pseudomonas aeruginosa;
Pfam search result
| Model | No. | Description | Position (aa) | Score | E-value
|
| Mur_ligase_M | 1 | Mur ligase middle domain | 116-301 | 96.9 | 1.3e-27
|
| Mur_ligase_C | 1 | Mur ligase family, glutamate ligase domain | 323-391 | 35.0 | 1.2e-08
|
BLAST search result (top 10 hits)
| ID | Description | Score | E-value
|
| WP_171033153.1 | MULTISPECIES: UDP-N-acetylmuramoyl-L-alanine--D-glutamate li...
| 2264 | 0.0e+00 |
| WP_095933708.1 | UDP-N-acetylmuramoyl-L-alanine--D-glutamate ligase [Streptom...
| 2110 | 0.0e+00 |
| WP_189187751.1 | UDP-N-acetylmuramoyl-L-alanine--D-glutamate ligase [Streptom...
| 2110 | 0.0e+00 |
| WP_165266697.1 | UDP-N-acetylmuramoyl-L-alanine--D-glutamate ligase [Streptom...
| 2104 | 0.0e+00 |
| WP_168490600.1 | MULTISPECIES: UDP-N-acetylmuramoyl-L-alanine--D-glutamate li...
| 2098 | 0.0e+00 |
| WP_037668865.1 | UDP-N-acetylmuramoyl-L-alanine--D-glutamate ligase [Streptom...
| 2096 | 0.0e+00 |
| GHJ98340.1 | UDP-N-acetylmuramoylalanine--D-glutamate ligase [Streptomyces sp...
| 2095 | 0.0e+00 |
| WP_152893965.1 | UDP-N-acetylmuramoyl-L-alanine--D-glutamate ligase [Streptom...
| 2094 | 0.0e+00 |
| WP_200416934.1 | UDP-N-acetylmuramoyl-L-alanine--D-glutamate ligase [Streptom...
| 2091 | 0.0e+00 |
| WP_201049015.1 | UDP-N-acetylmuramoyl-L-alanine--D-glutamate ligase [Streptom...
| 2087 | 0.0e+00 |
PSORT result
| Localization | Certainty | PSORT said
|
| membrane | 0.1171 | Seems to have an uncleavable N-term signal seq
|