| ID | SAVERM_7340
| Type | CDS
|
| From | 8756214
| nt length | 1323
|
| To | 8754892
| aa length | 440
|
| Gene | egtB
| pI | 5.21
|
| Definition | putative formylglycine-generating sulfatase
| M.W (Da) | 49189
|
| Note | biosynthetic gene cluster for ergothioneine, PF03781: Formylglycine-generating sulfatase enzyme
| AseI frg. | D
|
| SignalP v.4.0 | Non-secretory protein | plot
| Category | similar to unknown proteins
|
Orthologs (if any)
| Bacteria* | Reciprocally best hit to | Score | E-value
|
| coel | SCO0911 hypothetical protein | 741 | 0.0e+00
|
| sgr | SGR_696 conserved hypothetical protein | 677 | 0.0e+00
|
| sam | SAM23877_6629 Iron(II)-dependent oxidoreductase EgtB | 731 | 0.0e+00
|
| scab | SCAB11221 hypothetical protein | 729 | 0.0e+00
|
| sace | SACE_3825 sulfatase modifying factor | 566 | 0.0e+00
|
| nfar | nfa48860 hypothetical protein | 593 | 9.5e-171
|
| mtub | gi|15610839 hypothetical protein Rv3703c [Mycobacterium tuberculosis H37Rv] | 435 | 2.5e-123
|
| bsub | BG12958 yfmG; hypothetical protein | 93 | 2.1e-20
|
| paer | PA2395 [gene=PA2395] [prot=hypothetical protein] [comment=PA2395] | 50 | 2.4e-07
|
*coel, Streptomyces coelicolor A3(2); sgr, Streptomyces griseus IFO13350; sam, Streptomyces ambofaciens ATCC 23877; scab, Streptomyces scabies 87.22; sace, Saccharopolyspora erythraea NRRL2338; nfar, Nocardia farcinica IFM 10152; mtub, Mycobacterium tuberculosis H37Rv; bsub, Bacillus subtilis; paer, Pseudomonas aeruginosa;
Pfam search result
| Model | No. | Description | Position (aa) | Score | E-value
|
| FGE-sulfatase | 1 | Sulfatase-modifying factor enzyme 1 | 178-436 | 226.4 | 4e-67
|
| DinB_2 | 1 | DinB superfamily | 20-151 | 95.1 | 4.3e-27
|
BLAST search result (top 10 hits)
| ID | Description | Score | E-value
|
| WP_153287459.1 | ergothioneine biosynthesis protein EgtB [Streptomyces fagopy...
| 1826 | 0.0e+00 |
| WP_168531368.1 | ergothioneine biosynthesis protein EgtB [Streptomyces sp. RP...
| 1814 | 0.0e+00 |
| WP_148009679.1 | ergothioneine biosynthesis protein EgtB [Streptomyces sp. me...
| 1813 | 0.0e+00 |
| QIY99910.1 | ergothioneine biosynthesis protein EgtB [Streptomyces sp. S1D4-11...
| 1807 | 0.0e+00 |
| WP_190168443.1 | ergothioneine biosynthesis protein EgtB [Streptomyces mirabi...
| 1806 | 0.0e+00 |
| WP_054231627.1 | MULTISPECIES: ergothioneine biosynthesis protein EgtB [uncla...
| 1804 | 0.0e+00 |
| WP_097223813.1 | ergothioneine biosynthesis protein EgtB [Streptomyces sp. OV...
| 1799 | 0.0e+00 |
| WP_095851159.1 | ergothioneine biosynthesis protein EgtB [Streptomyces sp. Ag...
| 1796 | 0.0e+00 |
| WP_189185586.1 | ergothioneine biosynthesis protein EgtB [Streptomyces albifl...
| 1794 | 0.0e+00 |
| WP_067360717.1 | ergothioneine biosynthesis protein EgtB [Streptomyces olivoc...
| 1793 | 0.0e+00 |
PSORT result
| Localization | Certainty | PSORT said
|
| cytoplasm | 0.2636 | Seems to have no N-terminal signal seq.
|