| ID | SAVERM_947
| Type | CDS
|
| From | 1206711
| nt length | 900
|
| To | 1205812
| aa length | 299
|
| Gene | aveBIII
| pI | 5.18
|
| Definition | glucose-1-phosphate thymidyltransferase
| M.W (Da) | 32450
|
| Note | avermectin gene cluster
| AseI frg. | A
|
| SignalP v.4.0 | Non-secretory protein | plot
| Category | antibiotic production
|
Orthologs (if any)
| Bacteria* | Reciprocally best hit to | Score | E-value
|
| sgr | SGR_637 putative glucose-1-phosphate thymidyltransferase | 401 | 1.0e-142
|
| sam | SAM23877_7104 putative glucose-1-phosphate thymidylyltransferase | 442 | 7.0e-159
|
| scab | SCAB84031 putative glucose-1-phosphate thymidyltransferase | 343 | 2.0e-119
|
| sace | SACE_6883 glucose-1-phosphate thymidylyltransferase | 420 | 4.0e-150
|
| nfar | nfa2070 rfbA glucose-1-phosphate thymidyltransferase | 331 | 4.1e-92
|
| mtub | gi|15607475 ALPHA-D-GLUCOSE-1-PHOSPHATE THYMIDYLYLTRANSFERASE RMLA (DTDP-GLUCOSE SYNTHASE) (DTDP-GLUCOSE PYROPHOSPHORYLASE) [Mycobacterium tuberculosis H37Rv] | 322 | 1.9e-89
|
| cglu | gi|19551581 COG1209:dTDP-glucose pyrophosphorylase [Corynebacterium glutamicum] | 322 | 1.0e-89
|
| bsub | BG10617 spsI, ipa-71d; spore coat polysaccharide biosynthesis protein spsI [EC:2.7.7.24] | 189 | 1.7e-49
|
| ecoli | gi|16129979 dTDP-glucose pyrophosphorylase [Escherichia coli K12] | 336 | 9.8e-94
|
| paer | PA5163 [gene=rmlA] [prot=glucose-1-phosphate thymidylyltransferase] [comment=PA5163] | 336 | 1.8e-93
|
*sgr, Streptomyces griseus IFO13350; sam, Streptomyces ambofaciens ATCC 23877; scab, Streptomyces scabies 87.22; sace, Saccharopolyspora erythraea NRRL2338; nfar, Nocardia farcinica IFM 10152; mtub, Mycobacterium tuberculosis H37Rv; cglu, Corynebacterium glutamicum; bsub, Bacillus subtilis; ecoli, Escherichia coli K-12; paer, Pseudomonas aeruginosa;
Pfam search result
| Model | No. | Description | Position (aa) | Score | E-value
|
| NTP_transferase | 1 | Nucleotidyl transferase | 2-235 | 197.5 | 2.5e-58
|
| NTP_transf_3 | 1 | MobA-like NTP transferase domain | 3-139 | 38.6 | 1.2e-09
|
BLAST search result (top 10 hits)
| ID | Description | Score | E-value
|
| WP_103504657.1 | glucose-1-phosphate thymidylyltransferase RfbA [Streptomyces...
| 1084 | 5.2e-147 |
| WP_103528603.1 | MULTISPECIES: glucose-1-phosphate thymidylyltransferase RfbA...
| 1078 | 4.8e-146 |
| WP_159503168.1 | glucose-1-phosphate thymidylyltransferase RfbA [Streptomyces...
| 1077 | 6.8e-146 |
| WP_191066673.1 | glucose-1-phosphate thymidylyltransferase RfbA [Streptomyces...
| 1073 | 3.3e-145 |
| WP_044567920.1 | glucose-1-phosphate thymidylyltransferase RfbA [Streptomyces...
| 1071 | 5.7e-145 |
| WP_198269562.1 | glucose-1-phosphate thymidylyltransferase RfbA [Streptomyces...
| 1070 | 7.4e-145 |
| WP_053143136.1 | glucose-1-phosphate thymidylyltransferase RfbA [Streptomyces...
| 1070 | 9.4e-145 |
| WP_121825753.1 | glucose-1-phosphate thymidylyltransferase RfbA [Streptomyces...
| 1070 | 9.7e-145 |
| WP_121010022.1 | glucose-1-phosphate thymidylyltransferase RfbA [Saccharothri...
| 1067 | 2.4e-144 |
| WP_210946957.1 | glucose-1-phosphate thymidylyltransferase RfbA [Streptomyces...
| 1067 | 2.4e-144 |
PSORT result
| Localization | Certainty | PSORT said
|
| membrane | 0.1319 | Seems to have no N-terminal signal seq.
|