SAVERM

ID SAVERM_948 Type CDS
From 1206837 nt length 1032
To 1207868 aa length 343
Gene aveBIV pI 8.12
Definition dTDP-4-keto-6-deoxy-L-hexose 4-reductase M.W (Da) 35630
Note avermectin gene cluster AseI frg. A
SignalP v.4.0 Non-secretory protein | plot Category antibiotic production

Orthologs (if any)

Bacteria*	Reciprocally best hit to	Score	E-value
sgr	SGR_636 putative deoxyhexose reductase	224	1.0e-71
sam	SAM23877_5606 putative NDP-hexose ketoreductase	229	8.0e-74
sace	SACE_0720 dTDP-4-keto-6-deoxy-L-hexose 4-reductase	272	1.0e-90
paer	PA3146 [gene=wbpK] [prot=probable NAD-dependent epimerase/dehydratase WbpK] [comment=PA3146]	65	5.3e-12

*sgr, Streptomyces griseus IFO13350; sam, Streptomyces ambofaciens ATCC 23877; sace, Saccharopolyspora erythraea NRRL2338; paer, Pseudomonas aeruginosa;

Pfam search result

Model	No.	Description	Position (aa)	Score	E-value
Epimerase	1	NAD dependent epimerase/dehydratase family	29-266	112.7	1.8e-32
RmlD_sub_bind	1	RmlD substrate binding domain	29-339	35.1	7.1e-09
NAD_binding_10	1	NAD(P)H-binding	33-184	28.4	1.2e-06
3Beta_HSD	1	3-beta hydroxysteroid dehydrogenase/isomerase family	30-250	22.2	5.6e-05

BLAST search result (top 10 hits)

ID	Description	Score	E-value
WP_210867748.1	NAD(P)-dependent oxidoreductase [Streptomyces sp. RG38] >lcl...	835	1.9e-107
WP_077967295.1	NAD-dependent epimerase/dehydratase [Streptomyces tsukubensi...	805	3.8e-103
WP_030916386.1	NAD-dependent epimerase/dehydratase family protein [Streptos...	798	3.1e-102
WP_151508728.1	NAD-dependent epimerase/dehydratase family protein [Streptom...	791	6.0e-101
WP_069930860.1	NAD-dependent epimerase/dehydratase family protein [Streptom...	773	1.5e-98
WP_069933793.1	NAD-dependent epimerase/dehydratase family protein [Streptom...	772	2.7e-98
WP_190191437.1	NAD-dependent epimerase/dehydratase family protein [Streptom...	768	1.2e-97
MXG26078.1	NAD-dependent epimerase/dehydratase family protein [Streptomyces ...	763	9.8e-97
WP_225976800.1	NAD-dependent epimerase/dehydratase family protein [Streptom...	761	1.7e-96
WP_237492162.1	NAD-dependent epimerase/dehydratase family protein [Streptom...	751	4.3e-95

PSORT result

Localization	Certainty	PSORT said
membrane	0.2084	Seems to have no N-terminal signal seq.

ID	SAVERM_948	Type	CDS
From	1206837	nt length	1032
To	1207868	aa length	343
Gene	aveBIV	pI	8.12
Definition	dTDP-4-keto-6-deoxy-L-hexose 4-reductase	M.W (Da)	35630
Note	avermectin gene cluster	AseI frg.	A
SignalP v.4.0	Non-secretory protein \| plot	Category	antibiotic production